site n. 1.地點(diǎn);位置;地基。 2.場(chǎng)所,現(xiàn)場(chǎng)。 3.遺址 ...
insertion n. 1.插入;記入;刊登。 2.插入物;插入句;插入廣告;插銹,補(bǔ)繡。 3.【動(dòng)、植】著生(點(diǎn))。 4.【電學(xué)】嵌入,介入。 5.【醫(yī)學(xué)】(肌肉的)附著。 the insertion of muscle 肌附著,肌止端。 adj. -al
site n. 1.地點(diǎn);位置;地基。 2.場(chǎng)所,現(xiàn)場(chǎng)。 3.遺址。 4.【計(jì)算機(jī)】網(wǎng)站,站點(diǎn)〔電腦網(wǎng)絡(luò)用戶的網(wǎng)站地址〕;萬(wàn)維網(wǎng)址(=Website)。 construction site 建筑工地。 firing [launching] site (火箭等)發(fā)射場(chǎng)。 historic sites 歷史遺址。 nuclear test site 核試驗(yàn)場(chǎng)。 the site of a battle 戰(zhàn)場(chǎng)。 vt. 給與位置,為…選定地點(diǎn);安放;使坐落于(某處)。 adj. -ed 地點(diǎn)[位置]…的 (a well-sited factory 地點(diǎn)好的工廠)。
The insertion sites of tn5gusa5 were located by methods of tail - pcr and sequencing . for these 53 protease mutants , the insertion sites were distributed in 14 orfs 運(yùn)用tail - por和測(cè)序的方法定位了轉(zhuǎn)座子tn5gusa5的插入位置,經(jīng)分析共插入14個(gè)orf中。
After amplifying genomic sequences flanked with tn5gusa5 by tail pcr and dna sequencing , then doing blast with genomic sequence of xcc 8004 , insertion sites of 10981 mutants were located precisely in xcc 8004 genome 經(jīng)tail - pcr擴(kuò)增tn5gusa5旁側(cè)序列并測(cè)序,經(jīng)與xcc8004基因組序列比對(duì)后獲得精確定位的tn5gusa5插入突變體10981個(gè)。
The technique " improed coordinated timing of mechanical actiation of papillary muscle insertion sites and the remote decrease of functional mitral regurgitation secondary to left entricular reerse remodeling , " the authors write 作者指出,這一技術(shù)提高了乳頭肌插入部機(jī)械出發(fā)和遠(yuǎn)隔部位二尖瓣重構(gòu)導(dǎo)致的二尖瓣功能降低性返流之間的協(xié)同考慮。
Based on the blast analysis and other studies , osddl mutant was a multi - copy - insertion mutant , and one of the insertion sites was in an nptii like transposase gene , whereas osdd2 , a single - copy - insertion mutant was disrupted at a gene encoded wrky transcript factor Osdd1突變體可能是多拷貝插入,其中一個(gè)插入到nptii轉(zhuǎn)座酶類似基因。 osdd2突變體為單拷貝、插入到一個(gè)wrky類轉(zhuǎn)錄因子基因5翻譯起始區(qū)附近。
The results showed that the f fragment , 728bp in length , could be a new gene with a little homology to the genes coding for polyketide synthetase or fatty - acid synthetase and the b fragment , about 4kb in length , is inferred to have repeat sequences around tn5 insertion site , in which there is homology to the wa 314 right arm of the high - pathogeniciry island of yersinia enterocolitica . to reveal any pathogenicity of enterobacter cloacae b8 and its mutated strains b8b and b8f to animals , the experiment with mice was carried out 結(jié)果顯示, f片段長(zhǎng)度為728bp ,與現(xiàn)有生物數(shù)據(jù)庫(kù)的blast比較分析,發(fā)現(xiàn)該序列僅有局部短于1oobp的區(qū)域與polyketide合成酶基因或與脂肪酸合成酶基因有低的同源性,推測(cè)為一新基因; b片段長(zhǎng)約4kb ,序列拼接結(jié)果推測(cè)靠近tn5插入位點(diǎn)部位有重復(fù)序列,對(duì)b片段tn5遠(yuǎn)端的部分序列進(jìn)行blast比較,發(fā)現(xiàn)它與小腸結(jié)腸炎耶爾森氏菌的強(qiáng)毒力島有一定的同源性。
And the two pah ' s of pcr primers that bind to the adapter and the sequence of f fragment close by tn5 respectively were also designed . the genomic dna of b8 was isolated , digested with bamh i , and ligated to the adapter . using the two pairs of the primers , two rounds of pcr were performed hi turn and a fragment of 239bp was amplified successfully . lt was proved by cloning and sequencing that 18bp of the fragment is the sequence opposite to f fragment on the left of tn5 insertion site in b8f , the other is part of the 728 bp of f fragment . this result makes it possible to continue to carry out chromosome walking , to clone and sequence the whole genes of b fragment and f fragment , and to reveal the antagonistic molecular mechanism of b8 試驗(yàn)研究設(shè)計(jì)并合成了由40和44個(gè)堿基的寡聚脫氧核苷酸組成的染色體爬行接頭,在接頭序列和測(cè)定的f片段近tn5的序列上,設(shè)計(jì)了2對(duì)染色體爬行用的pcr引物,從b8菌株中提取基因組dna , bamhi酶切,與染色體爬行接頭連接,依次用2對(duì)引物進(jìn)行pcr ,擴(kuò)增出239bp產(chǎn)物,經(jīng)克隆、測(cè)序,發(fā)現(xiàn)其中18bp為擴(kuò)增的相應(yīng)于f片段在b8f菌株tn5插入位點(diǎn)對(duì)面的序列,其余則為f片段728bp序列的一部分,為進(jìn)一步進(jìn)行染色體爬行,克隆和測(cè)定整個(gè)b和f基因,揭示陽(yáng)菌株的拮抗分子機(jī)制提供了技術(shù)資料貯備。
In addition , molecular experiments indicated that resistance was not relevant to copy number or to insertion sites , but instead to the repeated gfp structure and to methylation of genomic dna . pvx vectors were constructed that contained the s6 gene of rbsdv vi and the 14 kd fragment of bnyvv rna2 分子檢測(cè)結(jié)果顯示, gfp3植株中普遍存在gfp基因的沉默現(xiàn)象,且gfpdna的甲基化程度明顯高于gfp1植株,說(shuō)明這些植株對(duì)于病毒的抗性與目的基因的插入位點(diǎn)及拷貝數(shù)和無(wú)關(guān),但與串聯(lián)重復(fù)的gfp基因結(jié)構(gòu)形式密切相關(guān)。